Er license from Isis Pharmaceuticals. glen unySuPPort frItS Universal Supports are the supports of choice for high throughput synthesis since they remove the need for individual wells or columns to contain a unique support. We now offer high density polyethylene orderIng InformatIon
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(HDPE) frits with embedded 500or 1000Glen UnySupport for high throughput O DMTO applications and to allow customers to make their own inexpensive O columns. O OMe The frits N H contain 40 nanomoles of Glen UnySupport O and fit directly into empty MerMade and OMe AB3900 columns. They can also be used on ABI 394 and Expedite instruments when fitted into inexpensive female-female luer adapters.
new clIck tagS Glen Research is collaborating with baseclick Gmbh to offer a variety of interesting alkyne phosphoramidites. We offer some azide products and are now embarking on an expansion of our azide catalog. Our strategy is to offer first our most popular labels for general interest and, subsequently, we will add azide products that are not compatible with phosphoramidite chemistry. Out first offering of azide tags for Click Chemistry, BiotinTEG, DesthiobiotinTEG, 6-FAM-TEG, and Dipivaloyl 6-FAM-TEG, is shown in Figure 1. Biotin is still our most commonly used label and biotinTEG, with its hydrophilic triethylene glycol spacer, is the most popular biotin product. Desthiobiotin is a biotin analogue that is well captured by streptavidin but the captured product can be easily released by applying a biotin solution to the streptavidin beads. 6-FAM is our most popular fluorescein derivative and we offer azides of both 6-FAM and pivaloylprotected 6-FAM for situations where subsequent reactions require the 6-FAM to be protected. In both 6-FAM products, the hydrophilic TEG spacer is again used. The azides are offered in 25 and 100 ole packs for convenient oligonucleotide labelling. Figure 2 illustrates the high efficiency of the click reaction between 6-FAM-TEG Azide and an oligonucleotide modified with C8-Alkyne-dT (10-1540) on a 0.8 ole scale. clIck Procedure Notes on Click Chemistry:
DEPROTEcTION VOLUME 5 ON-cOLUMN DEPROTEcTION Of OLIGONUcLEOTIDES IN ORGANIc SOLVENTS
The deprotection of oligonucleotides, especially for high-throughput syntheses, can be the rate-limiting step during the production of oligos and is often difficult to automate due to issues with liquid handling. To streamline the deprotection process, gas phase deprotection using ammonia or methylamine gas is often employed1. After the removal of the protecting groups is complete, the oligo is conveniently eluted directly in water or the buffer of choice. However, the equipment necessary to safely handle a pressurized, corrosive gas is expensive and the additional cost is not worthwhile for many smaller production facilities and research labs.393121-74-9 Synonym An alternative method that incorporates much of the convenience of gas-phase deprotection but still utilizes low-cost and simple equipment is On-Column deprotection.495-60-3 Formula In this case, the nucleophilic amine used to remove the protecting groups is dissolved in a non-polar solvent, such as toluene, in which the deprotected oligonucleotide is insoluble.PMID:30691991 After the deprotection of the oligonucleotide is complete, the column is rinsed, allowed to briefly dry and the oligo, still bound to the support, is eluted in the aqueous buffer of choice, as described by Kempe2. A similar strategy was used by Damha for the deprotection of RNA on glass slides3. Based upon a protocol used t.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
