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Name :
PCK2 Protein

Description :
PCK2 promotes tumor initiation by lowering acetyl-CoA level through reducing the mitochondrial tricarboxylic acid (TCA) cycle. The levels of phosphoenolpyruvate carboxykinase isoform 2 (PCK2) are critical for the metabolic switch and the maintenance of TICs in prostate cancer. PCK2 is a potential therapeutic target for aggressive prostate tumors.

Species :
Human

Uniprotkb :
Baculovirus-Insect Cells

Tag :
His,GST

Synonyms :
PEPCK-M, PEPCK2, PEPCK, phosphoenolpyruvate carboxykinase 2 (mitochondrial)

Construction :
A DNA sequence encoding the human PCK2 (NP_004554.3) (Leu33-Met640) was expressed with a C-terminal polyhistidine-tagged GST tag at the N-terminus (his-GST).

Protein Purity :
> 90 % as determined by SDS-PAGE.

Molecular Weight :
Approxiamtely 94.9 kDa

Endotoxin :

Formulatione :
Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 %glycerol, pH 8.0. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

Reconstitution :
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Stability & Storage :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping :
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.

Research Background :
PCK2 promotes tumor initiation by lowering acetyl-CoA level through reducing the mitochondrial tricarboxylic acid (TCA) cycle. The levels of phosphoenolpyruvate carboxykinase isoform 2 (PCK2) are critical for the metabolic switch and the maintenance of TICs in prostate cancer. PCK2 is a potential therapeutic target for aggressive prostate tumors.

References and Literature :

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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Author: HMTase- hmtase