Share this post on:

Product Name :
Biotinylated Human 4-1BB Ligand Protein

EN
41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9

Description:
| Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein with the N-monomeric hFc Tag |Source: The reco|The reco|>95% by SEC-HPLC and SDS-|Less than 1 EU per ug of protein as determined by LAL method. |Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |Reconstitution: It is recommended to reconstitute the lyophilized reco|The recombinant Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein is shipped with ice |Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.

Source:
The recombinant Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein, (amino acids Arg71-Glu254) fused with a monomeric hFc tag at the N-terminus, was produced in HEK 293 cells.

Molecular Weight :
The recombinant Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer protein with the monomeric hFc tag at the N-terminus has a predicted MW of 84.3 kDa same as Tris-Bis PAGE result.

Activity:
Immobilized recombinant Human 4-1BB protein with the N-monomeric hFc tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human 4-1BB Ligand (Trimer) protein with the N-hFc tag with the EC50 of 27.1 ng/ml determined by ELISA.

Purity :
>95% by SEC-HPLC and SDS-PAGE under reducing conditions.

Endotoxin:
LevelLess than 1 EU per ug of protein as determined by LAL method.

Formulation :
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution:
It is recommended to reconstitute the lyophilized recombinant Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

Shipping:
The recombinant Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage :
/strong>Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
1948273-02-6 InChIKey 353-09-3 InChIKey PMID:26574782 MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com

Share this post on:

Author: HMTase- hmtase