Name :
Recombinant Mouse CES1 Protein (His Tag), HPLC-verified
Biological Activity :
Background :
CES1 encodes carboxylesterase-1, an important drug-metabolizing enzyme with high expression in the liver. Variants generated by the exchange of segments with CES1P1 complicate the genotyping of CES1. CES1 is involved in the hydrolysis of ester group-containing xenobiotic and endobiotic compounds including several essential and commonly used drugs. The individual variation in the efficacy and tolerability of many drugs metabolized by CES1 is considerable. However, it has been problematic to express recombinant CES1 in bacterial expression systems due to low solubility, with the CES1 protein being mainly expressed in inclusion bodies, accompanied by insufficient purity issues. CES1 is capable of catalyzing the hydrolysis of a wide range of therapeutic agents, toxins, and endogenous compounds. Accumulating studies have demonstrated associations between the expression and activity of CES1 and the pharmacokinetics and/or pharmacodynamics of CES1 substrate medications.
Biological Activity :
Measured by its ability to hydrolyze p-nitrophenylacetate.
Expression Host :
Mouse
Source :
HEK293 Cells
Tag :
Protein Accession No. :
Q8VCC2
NCBI Gene ID :
Synonyms :
Synonyms :
carboxylesterase 1
Amino Acid Sequence :
Molecular Weight :
The recombinant mouse CES1 consists of 558 amino acids and predicts a molecular mass of 62.25 kDa. It migrates as an approximately 57.32 kDa band in SDS-PAGE under reducing conditions.
Purity :
≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the mouse CES1 (Q8VCC2) (Met1-Leu565) was expressed with a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
References & Citations :
Sanford JC, et al. (2016) Regulatory effects of genomic translocations at the human carboxylesterase-1 (ces1) gene locus. Pharmacogenet Genomics 26 (5): 197-207.Rasmussen HB, et al. (2015) Individualization of treatments with drugs metabolized by ces1: Combining genetics and metabolomics. Pharmacogenomics 16 (6): 649-665.Qian Y, et al. (2020) Natural products as modulators of ces1 activity. Drug Metab Dispos 48 (10): 993-1007.Ferrero-Miliani L, et al. (2017) Reappraisal of the genetic diversity and pharmacogenetic assessment of ces1. Pharmacogenomics 18 (13): 1241-1257.
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