The MS spectra from the analytes and those of standards there was a good match of your RT values and exact mz ratios with the [MH] and [MH] ions (Tables and) at the same time as of your MS spectra from the [MH] ions (Tables and).Frontiers in Plant Science www.frontiersin.orgNovember Volume ArticleSisTerraza et al.Coumarins in FeDeficient Arabidopsis PlantsFIGURE Chromatographic separation of a range of phenolictype compounds created in Sutezolid manufacturer response to Fe deficiency by Arabidopsis thaliana roots.Typical fluorescence (at exc and em nm) and absorbance (at nm) chromatograms for root and development media extracts from plants grown as described in Fourcroy et al. plants have been pregrown for days inside the presence of Fe (III)EDTA at pH after which grown for days in a medium with (Fe) or Fe (III)EDTA (Fe) (the pH was not readjusted to with the final pH becoming c..in all pots).Chromatograms were obtained utilizing Elution plan .The encircled numbers above each and every peak correspond to the phenolic compounds listed in Table .RU, relative units, AU, absorbance units, and RT, retention time.Four more compounds have been initial confirmed to become hexosidetype compounds in the RT, exact mz values and MS spectra of your [MH] ions.The RT values of these compounds (.min) were close to those of recognized coumarin glucosides (.and .min for scopolin and fraxin, respectively), and lower than those of coumarin aglycones (.min for fraxetin, scopoletin, isofraxidin and fraxinol), phenylpropanoids (e.g .and .min for ferulic acid and sinapyl aldehyde), and glycoside and aglycone forms of other phenolics (e.g min for flavonoids, stilbenes and lignans) (Supplementary Figures S and S).For that reason, the RTs indicate that compounds are most likely to become polar (i.e hexoside) forms of coumarins andor phenylpropanoids.Additionally, inside the MS(TOF) spectra, ions (positivenegative) at mz .and .for , , , and , respectively, had been constant with the loss of a hexosyl moiety (.Da) from their corresponding [MH] [MH] ions (see mz values in Table).This was confirmed making use of the low resolution MS spectra obtained together with the ion trap key fragment ions (relative intensity at mz , , and inside the MS spectra of , respectively; Table) corresponded to the [MH] ions (mz , , and for , , , and , respectively) after a mass loss of Da.Precisely the same mass loss was also observed inside the MS spectra of authenticated standards with the coumarin glucosides scopolin and fraxin described above, with important ions at mz (scopolin) and (fraxin), corresponding with the mz of their aglycones, scopoletin andfraxetin, respectively (Table).The rest of ions inside the MS spectra of compounds , scopolin and fraxin showed considerably reduced relative intensities , indicating the hexosyl loss is favored.The aglycon moieties of compounds had been identified taking benefit of possessing the dehexosylated ions in the MS(TOF) spectra and also carrying out low resolution MS experiments on the ion trap.Initially, from the positive and damaging MS(TOF) spectra, the mz values for dehexosylated ions (see above) of , , , and had been assigned towards the elemental formulae C H O , C H O , C H O and C H O , respectively (with absolute errors ppm).Two of these elemental formulae, C H O and C H O , have been consistent with coniferyl and sinapyl aldehydes, involved in coumarin synthesis (Kai et al) (Table), whereas the other two, C H O and C H O , were constant with two coumarins already identified within the samples (compounds PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21541725 and , respectively) (Table).Ultimately, compounds had been confirmed.
