Ons Thomas JR Frith, Conceptualization, Sources, Formal evaluation, Investigation, Writing–review and editing; Ilaria Granata, Data curation, Formal evaluation, Investigation, Writing–review and editing; Matthew Wind, Erin Stout, Dylan Stavish, James OS Hackland, Stuart L Johnson, Marysia Placzek, Investigation; Oliver Thompson, Paul R Heath, Investigation, Writing–review and editing; Katrin Neumann, Daniel Ortmann, Resources; 7-Ethoxyresorufin Metabolic Enzyme/Protease Konstantinos Anastassiadis, Resources, Writing–review and editing; Mina Gouti, Funding acquisition, Investigation, Writing–review and editing; James Briscoe, Valerie Wilson, Funding acquisition, Writing–review and editing; Mario R Guarracino, Peter W Andrews, Supervision, Funding acquisition, Writing–review and editing; Anestis Tsakiridis, Conceptualization, Sources, Formal analysis, Supervision, Funding acquisition, Investigation, Writing– original draft, Project administration, Writing–review and editing Author ORCIDs Thomas JR Frith https://orcid.org/0000-0002-6078-5466 James OS Hackland https://orcid.org/0000-0001-7087-9995 Konstantinos Anastassiadis http://orcid.org/0000-0002-9814-0559 James Briscoe http://orcid.org/0000-0002-1020-5240 Valerie Wilson http://orcid.org/0000-0003-4182-5159 Anestis Tsakiridis http://orcid.org/0000-0002-2184-2990 Choice letter and Author response Decision letter https://doi.org/10.7554/eLife.35786.033 Author response https://doi.org/10.7554/eLife.35786.More filesSupplementary files . Supplementary file 1. Significantly up- and downregulated transcripts, GO enrichment and TF signatures from RNAseq analysisDOI: https://doi.org/10.7554/eLife.35786.Supplementary file two. List of genes upregulated in unique NC populations and GO enrichment analysis.DOI: https://doi.org/10.7554/eLife.35786..Supplementary file 3. List of transcription variables shared between diverse NC populationsDOI: https://doi.org/10.7554/eLife.35786.Supplementary file 4. List of all genes up- and down-regulated in indicated NC populations and their progenitors..DOI: https://doi.org/10.7554/eLife.35786..Supplementary file five. List of primers Transparent reporting formDOI: https://doi.org/10.7554/eLife.35786..DOI: https://doi.org/10.7554/eLife.35786.Information availability The microarray and RNAseq data have already been deposited to GEO (GSE109267 and GSE110608). The following datasets were generated:Database, license, and accessibility information Publicly obtainable at the NCBI Gene Expression Omnibus (accession no: GSE10 9267)Author(s) Heath PRYear Dataset titleDataset URLhttps://www.ncbi.nlm. 2018 Axial progenitors produce trunk neural crest cells at a high efficiency nih.gov/geo/query/acc. cgi?acc=GSE109267 in vitroFrith et al. eLife 2018;7:e35786. DOI: https://doi.org/10.7554/eLife.21 ofResearch articleDevelopmental Biology Stem Cells and Regenerative Medicine Granata I, Tsakiridis 2018 RNA sequencing analysis of human https://www.ncbi.nlm. nih.gov/geo/query/acc. A embryonic stem cells and axial cgi?acc=GSE110608 progenitors Publicly offered at the NCBI Gene Expression Omnibus (accession no: GSE110608)
PCSK9 is often a proprotein convertase that acts as a negative regulator from the LDL receptor (Seidah et al., 2014). PCSK9 is synthesized mainly in hepatocytes and secreted into the bloodstream. Circulating PCSK9 binds towards the LDL receptor and diverts it to lysosomes for degradation, thereby leading to decreased LDL receptor abundance at the hepatocyte cell surface, decreased LDL clearance, and hypercholesterolem.
