Location of 12,491.72 hectares in the West Zone from the municipality of Rio de Janeiro [24]. For this reason, many initiatives had been proposed, aiming to mitigate the effects of human occupation in this environment, for instance the implementation of a biological station named Esta o Biol ica FIOCRUZ Mata Atl tica (EFMA: Fiocruz Atlantic Forest Biological Station). The EFMA is a part of the campus FIOCRUZ Mata Atl tica (CFMA–FIOCRUZ Atlantic Forest Campus), and is presently an environmentally protected area surrounded by low-income communities [257]. Within this location, quite a few scientific investigation projects have been created, which includes the monitoring of fauna [26] and its parasites [17,28]. In EFMA, infections by trypanosomatids have been described in distinct hosts, for example bats, dogs, marsupials, and humans [17,25,27,29]. Remarkably, two new Trypanosoma species had been described within this area–T. janseni and Trypanosoma caninum, [17,29]–showing that this location, although somewhat little, may possibly nonetheless present unknown trypanosomatid diversity. In this study, we evaluated trypanosomatid infections in rodents and marsupials collected inPathogens 2021, ten,three ofareas from EFMA with various habitat qualities as outlined by the level of anthropic influence. Infections had been detected, employing parasitological, molecular, and Methyl jasmonate In Vitro serological assays, and parasites have been identified by DNA sequence analysis. two. Final results 2.1. Tiny Mammals and Their Sampling Locations The species Didelphis aurita (Wied-Neuwied, 1826) broadly prevailed within the study location (n = 70), followed by Akodon cursor (Winge, 1887) (n = 7), Rattus rattus (Linnaeus, 1758) (n = 7), Marmosa paraguayana (Tate, 1931) (n = four), Oligoryzomys nigripes (Olfers, 1918) (n = two), Monodelphis americana (M ler, 1776) (n = 1), and Metachirus myosurus (Temminck, 1824) (n = 1). Probably the most captured species, D. aurita, was collected in all expeditions: 19 in July 2012, 11 in November 2012, 9 in April 2013, 15 in July 2013, 15 in November 2013, and five in April 2014, including the four recaptures. A significantly larger quantity of small mammals captured was observed in peridomicile region A1 (n = 51) than within the other locations; namely, transition region A2 (n = 32) and preserved forest location A3 (n = 11) (2 = 12.372, p = 1.2607E-05, df = two). 2.2. Infection Prices of Trypanosomatids In spite of the differences observed within the number of collected people, we didn’t observe a considerable distinction in trypanosomatid prevalence among the various environments: A1 (36/50, 72 , confidence interval: 57.53.7), A2 (23/30, 76.7 , CI: 57.70.1), and A3 (11/9, 81.8 , CI: 48.27.7) (2 = 0.07819, p = 0.96166, df = two) (Table 1). Seventy-five specimens of marsupials and sixteen specimens of rodents collected have been analyzed for trypanosomatids, totaling ninety-one JNJ-42253432 Technical Information individuals. Taking into consideration all the host species, the total trypanosomatid prevalence was 74.7 (CI: 64.53.three). Trypanosomatid prevalence was similar for marsupials (76 , CI: 64.75.1) and rodents (68.7 , CI: 41.38.9), with out substantial difference (two = 0.054569, p = 0.8153, df = 1). No important distinction was observed in trypanosomatid prevalence among male (73.6 , CI: 59.74.7) and female (76.3 , CI: 59.88.five) hosts (2 = 0.01261, p = 0.91059, df = 1).Table 1. Rodents and marsupials captured in three environments (peridomicile–A1, transition–A2, and preserved forest–A3) at EFMA, Rio de Janeiro (RJ), Brazil, among 2012 and 2014, and their infection rates by trypanosomatids. Order (n) Rodentia (16).
