Le as a potential anti-obesity herbal medicine.Supplementary Materials: The following
Le as a possible anti-obesity herbal medicine.Supplementary Components: The following are offered online at https://www.mdpi.com/article/10 .3390/ani11113187/s1: Figure S1. HPLC chromatograms of (A) morroniside and loganin standards, (B) Cornus officinalis extract, (C) 4-hydroxybenzoic acid (4-HBA) typical, and (D) Ribes fasciculatum extract; Figure S2. HPLC chromatograms of (A) of 4-hydroxybenzoic acid (4-HBA) and loganin standards and (B) CR extract. CR; Cornus officinalis and Ribes fasciculatum extract; Figure S3. (A) Physique weight modifications of mice throughout 12 weeks of ND offered with CR extract (300 mg/kg/day). (B) Total mouse body weight at the end of the experiment; Figure S4. Oral glucose tolerance test (OGTT) of ND mice provided CR extract (300 mg/kg/day) in 12 weeks. (A) Time course of blood glucose levels for the duration of the total glucose tolerance test. (B) Representative blood glucose levels at 15 min of glucose tolerance test; Figure S5. Insulin tolerance test (ITT) of ND mice provided with CR extract (300 mg/kg/day) in 12 weeks. (A) Time course of blood glucose levels in the course of the total insulin tolerance test. (B) Representative blood glucose levels at 45 min of insulin tolerance test; Figure S6. Effects of HFD-induced obesity on plasma profiles. Plasma levels of (A) aspartate aminotransferase (AST), (B) alanine aminotransferase (ALT), (C) triglyceride, and (D) total cholesterol had been examined employing DRI-CHEM NX500. ND; standard diet, HFD; high-fat diet, p 0.05 vs. ND (unpaired t-test); Figure S7. Comparison of metabolic profiles involving the ND and HFD groups. Mice had been fed ND or HFD for 12 weeks. Metabolic profiles with regards to (A) typical VO2 , (B) average VCO2 , (C) respiratory exchange ratio, and (D) power expenditure have been evaluated. VO2 ; average oxygen consumption, VCO2 ; typical carbon dioxide production. ND; standard diet plan, HFD; high-fat diet program. p 0.05 vs. ND (one-way ANOVA with Tukey’s honestly important difference post hoc test); Figure S8. Comparison of metabolic profiles involving the HFD and HFD CR300 groups. Mice had been fed HFD or HFD Animals 2021, 11,11 ofCR300 for 12 weeks. Average (A) meals intake and (B) drink consumption in the course of the metabolic cage analysis have been measured. HFD; high-fat diet. CR; CR extract administration; Table S1. Calibration curve parameters for 4-hydroxybenzoic acid and loganin; Table S2. Benefits of accuracy determination by MCC950 Technical Information analyzing loganin and 4-hydroxybenzoic acid at identified concentrations; Table S3. Outcomes of repeatability and intermediate precision. Author Tianeptine sodium salt Technical Information Contributions: S.-Y.J. and E.P. made the study; E.P., C.-G.L., H.J. (Hyoju Jeon), S.Y. and Y.Y. performed the experiments and analyzed the data; E.P., C.-G.L. and H.J. (Hyesoo Jeong) interpreted the information; E.P., C.-G.L. and S.-Y.J. wrote the paper; S.-Y.J. edited the manuscript. All authors have read and agreed to the published version with the manuscript. Funding: This study was supported by a grant from High Value-Added Meals Technology Development Plan, Ministry of Agriculture, Meals, and Rural Affairs (117041-03-1-SB010) along with a grant in the INNOPOLIS Foundation, Ministry of Science and ICT by the Korean government (2019-DD-RD-0088). Institutional Evaluation Board Statement: This study was authorized by the Institutional Animal Care and Use Committee (IACUC) of Ajou University College of Medicine (2020-0036) and carried out in accordance with the institutional recommendations. Data Availability Statement: The information presented within this study are readily available on request in the.
