Totally free details in English and Mandarin around the novel coronavirus COVID19. The COVID-19 resource centre is hosted on Elsevier Connect, the company’s public news and information and facts website.Elsevier hereby grants permission to create all its COVID-19-related investigation that may be accessible on the COVID-19 resource centre – such as this research content material – straight away readily available in PubMed Central as well as other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any type or by any means with acknowledgement from the original source. These permissions are granted at no cost by Elsevier for as long as the COVID-19 resource centre remains active.Chemico-Biological Interactions 346 (2021)Contents lists obtainable at ScienceDirectChemico-Biological Interactionsjournal homepage: www.elsevier.com/locate/chembiointComputational evaluation of TMPRSS2 expression in standard and SARS-CoV-2-infected human tissuesWenxiu Cao a, b, Qiushi Feng a, b, Xiaosheng Wang a, b, a bBiomedical Informatics Study Lab, College of Standard Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, 211198, China Large Information Study Institute, China Pharmaceutical University, Nanjing, 211198, ChinaA R T I C L E I N F OKeywords: SARS-CoV-2 Transmembrane serine protease two Immune signatures Gene expression profiles Pathway and gene ontology Gene co-expression networkA B S T R A C TThe transmembrane serine protease 2 (TMPRSS2) can be a key molecule for SARS-CoV-2 invading human host cells. To provide insights into SARS-CoV-2 infection of numerous human tissues and recognize the potential mechanism of SARS-CoV-2 infection, we investigated TMPRSS2 expression in a variety of regular human tissues and SARS-CoV2-infected human tissues. Employing publicly offered datasets, we performed computational analyses of TMPRSS2 expression levels in 30 typical human tissues, and compared them involving males and females and between younger (ages 49 years) and older (ages 49 years) populations in these tissues. We discovered that TMPRSS2 expression levels had been the highest within the prostate, stomach, pancreas, lungs, smaller intestine, and salivary gland. The TMPRSS2 protein had fairly high expression levels inside the parathyroid gland, stomach, smaller intestine, pancreas, kidneys, seminal vesicle, epididymis, and prostate. Nonetheless, TMPRSS2 expression levels had been not drastically different involving females and males or among younger and older populations in these tissues. The pathways enriched in TMPRSS2-upregulated pan-tissue were GLUT1 Inhibitor medchemexpress mainly involved in immune, metabolism, cell growth and proliferation, stromal signatures, and cancer as well as other diseases. Numerous cytokine genes displayed good expression correlations with TMPRSS2 in pan-tissue, which includes TNF-, IL-1, IL-2, IL-4, IL-7, IL-8, IL-12, IL18, IFN-, MCP-1, G-CSF, and IP-10. We further analyzed TMPRSS2 expression levels in nasopharyngeal swabs from SARS-CoV-2-infected patients. TMPRSS2 expression levels CLK Inhibitor Formulation showed no considerable difference in between males and females or in between younger and older sufferers. Nevertheless, they have been drastically reduce in SARS-CoV-2infected than in healthy folks and individuals with other viral acute respiratory illnesses. Interestingly, TMPRSS2 expression levels were positively correlated with all the enrichment levels of 4 immune signatures (B cells, CD8+ T cells, NK cells, and interferon response) in SARS-CoV-2-infected individuals but most likely to be negatively correlated with them.
