Tions NAO created the experimental strategy and plasmid design and style, MMP-9 Formulation performed cloning
Tions NAO developed the experimental tactic and plasmid design and style, performed cloning procedures, and drafted the manuscript. SVK performed the cell culture experiments and helped to draft the manuscript. IIV initiated the project, participated in its style and coordination and drafted the manuscript. NAO, JAH and IIV conducted the copy number determination experiments, SVK, JAH and IIV performed eGFP level determinations. AGG and KGS coordinated the project. All of the authors have read and approved the final manuscript. Acknowledgments The authors want to thank Dr Sergei Khaidukhov for performing the FACS analysis and Dr Ivan Vorobiev (senior) for delivering the suggestions for FACS information interpretation. We also thank Dr Ivan Zvyagin, Lev Usakin, Maria Kordyukova and Victoria Shender for taking aspect in the molecular cloning procedures. The function was supported by the following grants: RFBR 13-0440277- “Combinatorial chemistry and biology approaches to theranostics of many sclerosis”; RFBR 14-04-00647 ” Combinatorial biology approaches working with yeast show for development of biocatalysts de novo”; Program on the Presidium of your Russian Academy of Sciences No24 “Detection of Viral Antigens as possible Triggers of Autoimmune Neurodegeneration and Nanotechnologies and Nanomaterials”; Plan of the Presidium of Russian Academy of Sciences “Fundamental science for medicine” – grant “Features ofOrlova et al. BMC MMP Purity & Documentation Biotechnology 2014, 14:56 biomedcentral.com/1472-6750/14/Page 11 of18. Wajih N, Hutson SM, Owen J, Wallin R: Enhanced production of functional recombinant human clotting element IX by baby hamster kidney cells engineered to overexpress VKORC1, the vitamin K 2,3-epoxide-reducing enzyme in the vitamin K cycle. J Biol Chem 2005, 280(36):316031607. 19. Bebbington CR, Hentschel CC: The use of vectors depending on gene amplification for the expression of cloned genes in mammalian cells. In DNA Cloning. Volume IIIth edition. Edited by Glover D. San Diego: Academic; 1987:16388.doi:10.1186/1472-6750-14-56 Cite this article as: Orlova et al.: Improved elongation factor-1 alpha-based vectors for steady high-level expression of heterologous proteins in Chinese hamster ovary cells. BMC Biotechnology 2014 14:56.Submit your next manuscript to BioMed Central and take full advantage of:Hassle-free on the web submission Thorough peer evaluation No space constraints or colour figure charges Immediate publication on acceptance Inclusion in PubMed, CAS, Scopus and Google Scholar Investigation which can be freely accessible for redistributionSubmit your manuscript at biomedcentral.com/submit
Redox Biology 2 (2014) 273Contents lists readily available at ScienceDirectRedox Biologyjournal homepage: elsevier.com/locate/redoxResearch PaperMitochondria-targeted heme oxygenase-1 induces oxidative tension and mitochondrial dysfunction in macrophages, kidney fibroblasts and in chronic alcohol hepatotoxicitySeema Bansal, Gopa Biswas 1, Narayan G. Avadhani nThe Department of Animal Biology along with the Mari Lowe Center for Comparative Oncology, College of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USAart ic l e i nf oArticle history: Received 2 July 2013 Received in revised type 16 July 2013 Accepted 16 July 2013 Accessible online 23 July 2013 Keywords and phrases: Heme oxygenase-1 Mitochondrial targeting Cytochrome c Oxidase Heme aa3 content material ROS production Autophagya b s t r a c tThe inducible kind of Heme Oxygenase-1 (HO-1), a major endoplasmic reticulum (ER) associated heme protein, is.
