Vs. control group. n = 5?8. Click here for file [http://www.biomedcentral.
Vs. control group. n = 5?8. Click here for file [http://www.biomedcentral.com/content/supplementary/14712369-6-4-S1.doc]AcknowledgementsThis work was supported by CONACYT (#25441) given to MEIR and DGAPA (IN210201) given to JPCH.
BMC NephrologyResearch articleBioMed CentralOpen AccessAICAR chemical information hypothyroidism attenuates protein tyrosine nitration, oxidative stress and renal damage induced by ischemia and reperfusion: effect unrelated to antioxidant enzymes activitiesVer ica M Tenorio-Vel quez1,2, Diana Barrera3, Martha Franco2, Edilia Tapia2, Rogelio Hern dez-Pando4, Omar Noel Medina-Campos1 and Jos?Pedraza-Chaverri*Address: 1Facultad de Qu ica, Departamento de Biolog , Edificio B, Segundo Piso, Laboratorio 209, Universidad Nacional Aut oma de M ico (UNAM), Ciudad Universitaria, 04510, M ico, D.F., M ico, 2Departamento de Nefrolog , Instituto Nacional de Cardiolog “Ignacio Ch ez”, Juan Badiano #1, Col. Secci XVI, 14080, Tlalpan, M ico, D.F., M ico, 3Facultad de Medicina, Departamento de Farmacolog , Universidad Nacional Aut oma de M ico (UNAM), Ciudad Universitaria, 04510, M ico, D.F., M ico and 4Instituto Nacional de Ciencias M icas y Nutrici “Salvador Zubir “, Departamento de Patolog , 14000, M ico, D.F., M ico Email: Ver ica M Tenorio-Vel quez – [email protected]; Diana Barrera – [email protected]; Martha Franco – [email protected]; Edilia Tapia – [email protected]; Rogelio Hern dez-Pando – [email protected]; Omar Noel Medina-Campos – [email protected]; Jos?Pedraza-Chaverri* – [email protected] * Corresponding authorPublished: 07 November 2005 BMC Nephrology 2005, 6:12 doi:10.1186/1471-2369-6-Received: 25 June 2005 Accepted: 07 NovemberThis article is available from: http://www.biomedcentral.com/1471-2369/6/12 ?2005 Tenorio-Vel quez et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.AbstractBackground: It has been established that hypothyroidism protects rats against renal ischemia and reperfusion (IR) oxidative damage. However, it is not clear if hypothyroidism is able to prevent protein tyrosine nitration, an index of nitrosative stress, induced by IR or if antioxidant enzymes have involved in this protective effect. In this work it was explored if hypothyroidism is able to prevent the increase in nitrosative and oxidative stress induced by IR. In addition the activity of the antioxidant enzymes PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27488460 catalase, glutathione peroxidase, and superoxide dismutase was studied. Control and thyroidectomized (HTX) rats were studied 24 h of reperfusion after 60 min ischemia. Methods: Male Wistar rats weighing 380 ?22 g were subjected to surgical thyroidectomy. Rats were studied 15 days after surgery. Euthyroid sham-operated rats were used as controls (CT). Both groups of rats underwent a right kidney nephrectomy and suffered a 60 min left renal ischemia with 24 h of reperfusion. Rats were divided in four groups: CT, HTX, IR and HTX+IR. Rats were sacrificed and samples of plasma and kidney were obtained. Blood urea nitrogen (BUN) and creatinine were measured in blood plasma. Kidney damage was evaluated by histological analysis. Oxidative stress was measured by immunohistochemical localization of protein carbonyls and 4hydroxy-2-nonenal modified proteins. The pro.
