Product Name: H2AFX (938CT5.1.1) Monoclonal Antibody
Applications: WB, IHC-P
Reactivity: Human
Conjugation: Unconjugated
Host: Mouse
Sourcr: This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the C-terminal region of human H2AFX.
Clonality: Monoclonal
CAS NO: 118409-57-7
Product: PCI 29732
Isotype: IgG1
Concentration:
Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Synonyms: H2AX; H2A.X; H2A/X; Histone H2AX; Histone H2A.X; H2AFX
Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/23844263?dopt=Abstract
